Coding
MymTsfGFP

Part:BBa_K1980003:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)


MymT sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. The sfGFP has been seperated from the MymT by a short linker (GlySerGlySerGlySer) to allow both domains to fold.

Source

MymT is originally from Mycobacterium tuberculosis. We ordered it as codon optimised DNA from IDT.

References

Ben Gold, Haiteng Deng, Ruslana Bryk, Diana Vargas, David Eliezer, Julia Roberts, Xiuju Jiang, & Carl Nathan (2009) “Identification of a Copper-Binding Metallothionein in Pathogenic Mycobacteria” Nat Chem Biol. 2008 October ; 4(10): 609–616. doi:10.1038/nchembio.109.